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KMID : 0368019840070010003
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Journal of Soonchunhyang University
1984 Volume.7 No. 1 p.3 ~ p.11
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Electron Microscopic Study on the Glial Cells in the Degenerating Neuropil of the Brain
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양남길
안의태/고정식/박경호
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Abstract
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Structural variations of the neuroglial cells in the degenerating nervous tissues are points of arguements in the neuromorphological field. The short of ultrastructural information on the specific nuclei is one of the critical points in the study of neuroglial activities, because the exact readings of the ultrastructural elements are essential for the differentiation of glial target relationships. Having the experiences of extensive studies on the fundus striati nucleus (Nucleus accumbens septi) during the last 10 years, we have studied the glial-target relation hips following the surgical isolation of the fundus striati nucleus of cat. Surgery was pert e Hied by vertically inserting a stainless steel tube-knife, measuring 5mm in diameter; into the brain down to the base of the skull. The blood supply to the tissue cylinder was maintained through the vessels at the base of the brain. After the survival time of I, 2, 3, 7, 14 and 28 dais, cats were perfused with a mixture of 1% paraformaldehyde-l% glutaraldehyde in phosphate buffer (pH 7.4) and postfixed in osmium tetroxide solution. The ultrathin sections were counterstained with uranyl acetate-lead citrate, and were examined in a JEM 100CX electron microscope.
The results are as follow:
1. In the primary stage, the protoplasmic astroglial cells are activated throughout the neuropil. They fill the large area between neuronal elements, and separate the degenerating elements from the intact elements.
2. The astroglial cells attack and dissolve the degenerated elements:
3. The microglial cells increase in number and are activated.
4. In the later stage, around the capillaries, there accumulate many hypertropied pericytes,. activated brain macrophages, and the cells of transitional forms between pericytes and. macrophages.
5. Ameboid aicroglia or macrophages also can separate the altering elements from the: intact elements.
6. Oligodendroglia shows passive reaction of retracting its myelinating lamellae from degenerating axons.
7. Glial fibrosis actively spreading in the degenerating nervous tissue was suspected as the possible reason of loss of regenerative function in central neuronal cells.
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